Sterile Water

Sterile Water is a high-purity, sterile preparation of water for laboratory and pharmaceutical applications. It is produced through processes such as distillation, reverse osmosis, or deionization, followed by sterilization, typically via autoclaving or 0.1–0.22 µm filtration, to remove bacteria, endotoxins, and other contaminants. Depending on the grade, it may be further treated to be free of nucleases (DNase/RNase), proteases, and DEPC. Sterile Water is a fundamental reagent in research, serving as a solvent, diluent, and vehicle for a wide range of applications.

As a fundamental solvent, Sterile Water acts by dissolving or reconstituting solid or concentrated compounds to create liquid solutions suitable for use. For example, it serves as a vehicle to dilute or dissolve drugs for injection. When used in laboratory techniques, its high purity ensures that it does not introduce contaminants that could interfere with or degrade sensitive biological samples and reactions, such as those involving DNA and RNA.

• Molecular Biology: Preparation of buffers, reagents, and media for techniques like PCR, qPCR, electrophoresis, cloning, and sequencing, particularly when nuclease-free.
• Cell Culture: As a component in the formulation of cell culture media and for the preparation of sterile solutions.
• Biochemistry: Dissolving and diluting peptides, proteins, and other biochemical reagents.
• General Laboratory Use: Washing, rinsing, and cleaning laboratory equipment and glassware.

CAS Number: 7732-18-5
PubChem CID: 962
Molecular Formula: H₂O
Molecular Weight: 18.02 g/mol
pH: 5.0 – 7.0
Purity: ≥ 99%
Form: Sterile liquid solution
Storage: Store at room temperature, protected from light and moisture
Solubility: Miscible with a wide range of polar solvents