TB500 (Thymosin B4 Acetate）

TB-500 is a synthetic research peptide derived from thymosin beta-4 (Tβ4), a highly conserved 43-amino acid protein found in human and animal tissues. TB-500 peptide corresponds to the N-terminal acetylated fragment (Ac-LKKTETQ) of thymosin beta 4, representing the active site responsible for actin binding, cell migration, and wound healing. This Thymosin Beta 4 fragment is widely studied for its roles in tissue repair, angiogenesis, and inflammation regulation.

TB-500 exerts its biological effects primarily through actin cytoskeleton dynamics. It functions as a G-actin (globular actin) sequestering protein, regulating actin polymerization—a process essential for cell motility, structure, and division. This mechanism triggers a cascade of downstream effects including enhanced cell migration, angiogenesis (formation of new blood vessels), and attenuation of inflammatory responses. TB-500 also promotes endothelial cell differentiation, keratinocyte migration, and collagen deposition, while demonstrating anti-fibrotic properties through modulation of the Akt signaling pathway.

• Tissue Repair and Regeneration: Investigated for promoting repair of muscles, tendons, ligaments, skin, and heart tissue.
• Angiogenesis Studies: Employed to evaluate new blood vessel formation through upregulation of vascular endothelial growth factor (VEGF) expression.
• Wound Healing: Studied for enhancing keratinocyte migration, collagen deposition, and dermal tissue repair.
• Inflammatory Modulation: Used in preclinical models to assess reduction of inflammation and immune regulation.

CAS Number: 885340-08-9
Molecular Formula: C₃₈H₆₈N₁₀O₁₄
Molecular Weight: 889.01 g/mol
Sequence: Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln (Ac-LKKTETQ)
Purity: ≥ 98% (batch-dependent; refer to Certificate of Analysis)
Form: White to off-white lyophilized powder
Storage: Store at ≤ -20°C in a cool, dry place, protected from light and moisture
Solubility: Soluble in sterile water and DMSO; prepare working solutions per laboratory protocol