DSIP（Delta Sleep-Inducing Peptide）

Delta Sleep-Inducing Peptide (DSIP) is a naturally occurring nonapeptide first isolated in the 1970s from the cerebral venous blood of rabbits during electrically induced sleep. It is a single, non-glycosylated polypeptide chain consisting of nine amino acids with the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu. Originally investigated for its sleep-promoting properties, DSIP has since been recognized as a neuromodulator with pleiotropic physiological effects. The compound is widely studied in neurobiology research for its effects on sleep architecture, stress responses, and neuroprotection.

DSIP exerts its effects through multifaceted interactions with multiple neurotransmitter systems and intracellular signaling cascades. It potentiates GABA-activated currents in hippocampal and cerebellar neurons, enhancing GABAergic inhibition—a plausible mechanism for its sedative and anticonvulsant effects. DSIP also modulates the glutamatergic system, particularly through interaction with NMDA receptors. Additionally, it influences the endocrine system, affecting enzymatic activity of serotonin-N-acetyltransferase—a pineal enzyme with strong circadian rhythmicity. DSIP produces dose-dependent antinociceptive effects mediated via the opioid receptor system, and has demonstrated neuroprotective properties by protecting neurons from oxidative stress.

• Sleep Research: Investigated for its ability to promote delta (slow-wave) and spindle EEG patterns, enhancing the most restorative stage of sleep.
• Stress and HPA Axis Studies: Employed to study regulation of stress responses via the hypothalamic-pituitary-adrenal (HPA) axis.
• Neuroprotection: Studied for protecting neurons from oxidative stress, with potential applications in neurodegenerative disease research.
• Pain and Analgesia Research: Used to investigate antinociceptive effects mediated through opioid receptor pathways.
• Antioxidant Research: Explored for its antioxidant properties, including modulation of catalase and superoxide dismutase (SOD) activities.

CAS Number: 62568-57-4 (free base)
Molecular Formula: C₃₅H₄₈N₁₀O₁₅
Molecular Weight: 848.81 g/mol (free base)
Sequence: Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu (WAGGDASGE)
Purity: ≥ 99%
Form: Lyophilized powder
Storage: Store at ≤ -20°C in a cool, dry place, protected from light and moisture
Solubility: Soluble in sterile water; prepare working solutions per laboratory protocol